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Animal-model evidence consistently shows local effects: angiogenesis at the injury site (VEGF upregulation), growth-factor signaling (EGF, FGF), nitric oxide pathway modulation, and accelerated tendon-to-bone interface healing in rat Achilles transection studies

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Cells were permeabilized with 0.2% Triton X-100 and 1 mg/ml lysozyme, and probed with affinity-purified anti-GspD antibody at a 1:10 dilution in PBS buffer with 2% BSA