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COMSTAT analysis CLSM z-stack images were analyzed using COMSTAT image analysis software for quantification of specific parameters of the three-dimensional biofilm structure including bio-volume and DEAD/LIVE cell ratio ( ATP biofilm disruption assay O/N adjusted Candida cultures (5x10 6 cells/ml) were added to a black walled flat-bottomed 96-well plate (Grenier), followed by pre-warmed RPMI 1640, in a 1:10 ratio and incubated (20 rpm, 37°C)

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official who now leads the Center for Science in the Public Interest, told me