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In order to improve outcomes, biphasic scaffolds can be created with a softer upper layer containing chondrogenic factors (e.g., TGF-β, chondroitin sulfate) and a stiffer lower layer, often loaded with calcium phosphate, hydroxyapatite, or bone morphogenetic protein (BMP), to provide structural support of the above cartilage layer and to promote osteogenic tissue formation and boney integration

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Each vial is provided as a lyophilized powder for handling in an appropriate laboratory setting