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To examine the capacity of WT and Mda5 -/- HSCs to repopulate lethally irradiated recipients, we sorted and transplanted 100 LSK-SLAM HSCs from young or aged WT or Mda5 -/- mice together with aged-matched CD45.1 competitors into CD45.1 lethally irradiated recipients, followed by whole bone marrow competitive secondary transplantations

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This meant that there was a large therapeutic window, and a wide range of the agent’s efficacy (µg–ng), at any stage of the noxious course