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After a 5-h incubation in 5% CO 2 and 37°C, the media was replaced with 200 μl of the starvation media, in the presence of 10 μm GM6001, 0.5 μm PF573228, 2.5 or 5 μm PP2, 3 μm FRAX597, 40 μm Nitisinone or DMSO control where indicated

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Still, this data point is something to be aware of when designing research studies [6]