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For quantification of sulfur-containing metabolites in cultured cancer cells and mouse serum, 10 µl samples were extracted with 90 µl of ice-cold extraction solvent (80% methanol and 20% H 2 O) containing isotope-labelled internal standards (36 µM 13 C 2 15 N-GSH, 0.92 µM 13 C 4 15 N 2 -GSSG and 10 µM D 4 -cystine)

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Research in mammalian models demonstrates plasma GHK-Cu concentrations of approximately 200 ng/mL in young organisms, with these levels declining dramatically with age to around 80 ng/mL in aged specimens