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5-Amino-1MQ is a small-molecule competitive inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes the methylation of nicotinamide using S-adenosyl methionine (SAM)

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Images were analyzed using the “Adiposoft” plug-in software in ImageJ (NIH).[22] Briefly, images were converted to 8-bit images and scald to 0.366 microns per pixel (corresponding to 20× magnification on the Leica microscope)